CKB antibody

Catalog No. ABIN6939145

The Mouse Monoclonal anti-CKB antibody has been validated for IHC and StM. It is suitable to detect CKB in samples from Human.

Quick Overview for CKB antibody (ABIN6939145)

Target: CKB (Creatine Kinase, Brain (CKB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CKB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CPTC-CKB-2

Product Details anti-CKB Antibody

Specificity: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length CKB protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: SH-SY5Y, HEK-293 and Cerebellum.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Alternative Name: CKB

Molecular Weight: 42kDa

Gene ID: 1152

UniProt: P12277