EGFR antibody (AA 985-996)

Catalog No. ABIN6939283

This Mouse Monoclonal antibody specifically detects EGFR in WB. It exhibits reactivity toward Human.

Quick Overview for EGFR antibody (AA 985-996) (ABIN6939283)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB)

Clone: F4

Product Details anti-EGFR Antibody

Binding Specificity: AA 985-996

Purpose: EGFR (Epidermal Growth Factor Receptor) Antibody

Specificity: This MAb recognizes a protein of 170 kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide corresponding to aa 985-996 of human EGFR

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: A431 cells. Placenta, Breast, Colon or Bladder Carcinoma.

Known Application: Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background:

ERBB1, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone

A431 cells. Placenta, Breast, Colon or Bladder Carcinoma.

Molecular Weight: ~170kDa (wild type) and ~145kDa (vIII variant)

Gene ID: 1956, 488293

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins