TIGIT antibody (AA 22-141)

Catalog No. ABIN6939306

This Mouse Monoclonal antibody specifically detects TIGIT in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for TIGIT antibody (AA 22-141) (ABIN6939306)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: TIGIT-3106

Product Details anti-TIGIT Antibody

Binding Specificity: AA 22-141

Purpose: TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer) Antibody

Specificity: TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is a immuno-oncology target for therapy.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Tonsil or Prostate Carcinoma.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background:

T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain containing 9 (VSIG9), V-set and transmembrane domain containing 3 (VSTM3), Washington University Cell Adhesion Molecule (WUCAM)

Human tonsil or prostate carcinoma

Molecular Weight: 26kDa

Gene ID: 201633, 421750

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints