Estrogen Receptor alpha antibody (AA 129-312)

Catalog No. ABIN6939347

This anti-Estrogen Receptor alpha antibody is a Mouse Monoclonal antibody detecting Estrogen Receptor alpha in IHC, FACS and StM. Suitable for Human.

Quick Overview for Estrogen Receptor alpha antibody (AA 129-312) (ABIN6939347)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: ESR1-3559

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 129-312

Specificity: This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Purification: Purified by Protein A/G

Immunogen: Recombinant human ESR1 protein fragment (around aa129-312) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: MCF-7 cells. Breast or Endometrial Carcinoma (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Molecular Weight: ~67kDa

Gene ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly