CD15 antibody

Catalog No. ABIN6939471

The Mouse Monoclonal anti-CD15 antibody has been validated for FACS, IHC, IF and StM. It is suitable to detect CD15 in samples from Human.

Quick Overview for CD15 antibody (ABIN6939471)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: Leu-M1-same as MMA

Product Details anti-CD15 Antibody

Immunogen: U937 histiocytic cell line

Isotype: IgM kappa

Application Details

Application Notes:

Positive Control: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: FUT4

Background: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Molecular Weight: ~220kDa

Gene ID: 2526

UniProt: P22083