CD15 antibody

Catalog No. ABIN6939479

This Rabbit Polyclonal antibody specifically detects CD15 in FACS, IHC, IF and StM. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD15 antibody (ABIN6939479)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Product Details anti-CD15 Antibody

Immunogen: Purified human neutrophils

Isotype: IgG

Application Details

Application Notes:

Positive Control: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Known Application: Flow Cytometry (2-4 μg/million cells), Immunofluorescence (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (5-10 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: FUT4

Background: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Molecular Weight: ~220kDa

Gene ID: 2526

UniProt: P22083