HLA-DRA antibody

Catalog No. ABIN6939637

The Mouse Monoclonal anti-HLA-DRA antibody is suitable to detect HLA-DRA in samples from Human, Cow and Cat. It has been validated for FACS and IF.

Quick Overview for HLA-DRA antibody (ABIN6939637)

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivity: Human, Cow, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 169-1B5-2

Product Details anti-HLA-DRA Antibody

Purpose: HLA-DRA (MHC II) Antibody

Specificity: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen.It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human peripheral blood leukocytes

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Alternative Name: HLA-DRA

Background:

DR alpha chain, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DRA, HLA DRA1, HLA-DRA, HLADRA1, Major histocompatibility complex class II DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA

Daudi or HuT78 cells. Tonsil or lymph node., Ramos

Molecular Weight: 36kDa (α, chain) and 27kDa (β, chain)

Gene ID: 3122, 520048

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response