Recombinant HSPD1 antibody

Catalog No. ABIN6939671

The Rabbit Monoclonal anti-HSPD1 antibody is suitable to detect HSPD1 in samples from Human, Mouse, Rat, Chicken, Monkey, Hamster, Dog, Cow, Rabbit, Pig, Sheep and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for Recombinant HSPD1 antibody (ABIN6939671)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Hamster, Dog, Cow, Rabbit, Pig, Sheep, Guinea Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: HSPD1-2206R

Product Details anti-HSPD1 Antibody

Purpose: Recombinant HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Antibody

Specificity: Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/2206R, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length HSP60 protein

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Known Application: Western Blot (0.25-0.5 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1

Background:

60 kDa chaperonin, 60 kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma, Human Brain, Human Kidney, Human Pancreas, K-562

Molecular Weight: 60kDa

Gene ID: 3329, 595053

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity