CD46 antibody

Catalog No. ABIN6940027

The Mouse Monoclonal anti-CD46 antibody is suitable to detect CD46 in samples from Human and Baboon. It has been validated for FACS and IF.

Quick Overview for CD46 antibody (ABIN6940027)

Target: CD46

Reactivity: Human, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD46 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 169-1-E4-3

Product Details anti-CD46 Antibody

Purpose: CD46 (Membrane Cofactor Protein) Antibody

Specificity: Recognizes a protein of 52 kDa-58 kDa, identified as CD46 (also known as membrane cofactor protein, MCP). Its epitope is located on the four immunogenic short consensus repeat (SCR) domains at N-terminus of the protein. CD46 exists as many isoforms in a variety of tissues. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. CD46 functions as a C3b/C4b-binding glycoprotein that inhibits complement activation on host cells. It also serves as a measles virus receptor, an adherence factor for group Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria.

No Cross-Reactivity: Horse (Equine)

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: K562, U937, HL60, HeLa and Jurkat cells and human lymphocytes. Lymph node and tonsil.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD46

Target: CD46

Alternative Name: CD46

Background:

AHUS2, Complement membrane cofactor protein, MCP, Measles virus receptor, Membrane cofactor protein, MIC10, TLX, Trophoblast leucocyte common antigen, Trophoblast lymphocyte cross reactive antigen

HeLa and Jurkat cells and human lymphocytes. Lymph node and tonsil., HL60, K562, U937

Molecular Weight: 52-58kDa

Gene ID: 4179, 510402

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization