ORC1 antibody

Catalog No. ABIN6936127

Product Details anti-ORC1 Antibody

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORC1 antibody is un-conjugated

Application: ELISA, Coating (Coat)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human ORC1 protein

Clone: 7F6-1

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells.

Known Application: ELISA (For coating, order antibody without BSA)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ORC1

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Alternative Name: ORC1L (ORC1L Products)

Synonyms: CG10667 antibody, DmORC1 antibody, DmOrc1 antibody, DmOrc1p antibody, Dmel\\CG10667 antibody, ORC antibody, ORC1 antibody, dORC1 antibody, orc1 antibody, rDmORC antibody, ORC1L antibody, HSORC1 antibody, PARC1 antibody, orc1l antibody, xorc1 antibody, AA545195 antibody, MmORC1 antibody, Orc1l antibody, Origin recognition complex subunit 1 antibody, origin recognition complex subunit 1 antibody, origin recognition complex subunit 1 L homeolog antibody, origin recognition complex, subunit 1 antibody, Orc1 antibody, ORC1 antibody, orc1.L antibody

Background: The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation. Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle, however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells.

Molecular Weight: 120kDa

Gene ID: 4998

UniProt: Q13415

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA