PIP antibody (AA 41-146)

Catalog No. ABIN6935823

Product Details anti-PIP Antibody

Target: PIP (Prolactin-Induced Protein (PIP))

Binding Specificity: AA 41-146

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Specificity: It recognizes a protein of 15 kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50 % of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Purification: Purified by Protein A/G

Immunogen: Recombinant human GCDFP-15 protein fragment (around aa 41-146) (exact sequence is proprietary)

Clone: PIP-1571

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HepG2 cells. Breast or Pancreas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PIP

Target: PIP (Prolactin-Induced Protein (PIP))

Alternative Name: PIP (PIP Products)

Synonyms: GCDFP-15 antibody, GCDFP15 antibody, GPIP4 antibody, GP17 antibody, mSMGP antibody, prolactin induced protein antibody, PIP antibody, Pip antibody

Molecular Weight: 15kDa

Gene ID: 5304

UniProt: P12273