Recombinant RPA2 antibody

Catalog No. ABIN6940537

The Rabbit Monoclonal anti-RPA2 antibody is suitable to detect RPA2 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant RPA2 antibody (ABIN6940537)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RPA2-3140R

Product Details anti-RPA2 Antibody

Specificity: Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70 kDa, 32-34 kDa, and 11-14 kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length Replication protein A2 (RPA2) protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: MCF-7, HeLa or HepG2 cell lysates (WB). Human tonsil (IHC).

Known Application: Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA2

Molecular Weight: 32-34kDa

Gene ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA