Vimentin antibody

Catalog No. ABIN6940881

The Mouse Monoclonal anti-Vimentin antibody (Clone LN-6) (ABIN6940881) specifically detects Vimentin in IHC. The antibody is reactive with Human, Rat, Mouse, Pig, Cow, Cat, Rabbit and Sheep samples.

Quick Overview for Vimentin antibody (ABIN6940881)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Mouse, Pig, Cow, Cat, Rabbit, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: LN-6

Product Details anti-Vimentin Antibody

Purpose: Vimentin (Mesenchymal Cell Marker) Antibody

Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate.

Immunogen: Human thymic nuclear extract

Isotype: IgM, kappa

Application Details

Application Notes:

Positive Control: Jurkat cells, Sarcomas, Melanomas.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Background:

VIM

Jurkat cells, Melanomas., Sarcomas

Molecular Weight: 57-60kDa

Gene ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis