CD34 antibody

Catalog No. ABIN6941168

The Mouse Monoclonal anti-CD34 antibody is suitable to detect CD34 in samples from Human, Cynomolgus and Rhesus Monkey. It has been validated for FACS, IHC and IF.

Quick Overview for CD34 antibody (ABIN6941168)

Target: CD34

Reactivity: Human, Cynomolgus, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: QBEnd-10

Product Details anti-CD34 Antibody

Purpose: CD34 (Hematopoietic Stem Cell &Endothelial Marker) Antibody

Specificity: This MAb recognizes a single chain, transmembrane,heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidasebut sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.

No Cross-Reactivity: Cow (Bovine), Dog (Canine), Rat (Rattus), Sheep (Ovine)

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Detergent solubilized vesicular suspension prepared from human term placenta

Isotype: IgG1, lambda

Application Details

Application Notes:

Positive Control: Jurkat or KG-1 cells. Human tonsil or angiosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background:

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Jurkat or KG-1 cells. Human tonsilor angiosarcoma.

Molecular Weight: 90-110kDa

Gene ID: 947, 374990

UniProt: P28906