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CD34 antibody (CD34) Primary Antibody

CD34 Reactivity: Human, Cynomolgus, Rhesus Monkey FACS, IF, IHC, ISt, StM Host: Mouse Monoclonal QBEnd-10
Catalog No. ABIN6941168
$494.31
Plus shipping costs $45.00
100 μg ABIN6941168
100 μg ABIN6941168
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  • Target
    Reactivity
    Human, Cynomolgus, Rhesus Monkey
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This CD34 antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
    Specificity
    This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.
    No Cross-Reactivity
    Cow (Bovine), Dog (Canine), Rat (Rattus), Sheep (Ovine)
    Purification
    Purified by Protein A/G
    Immunogen
    Detergent solubilized vesicular suspension prepared from human term placenta
    Clone
    QBEnd-10
    Isotype
    IgG1, kappa
  • Application Notes

    Positive Control: Jurkat or KG-1 cells. Human tonsil or angiosarcoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Alternative Name
    CD34 (CD34 Antibody Abstract)
    Synonyms
    AU040960, CD34 molecule, CD34 antigen, CD34, Cd34
    Molecular Weight
    90-110kDa
    Gene ID
    947
    UniProt
    P28906
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