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CD34 antibody (CD34) Primary Antibody

CD34 Reactivity: Human, Rat FACS, IF, IHC, StM, WB Host: Mouse Monoclonal HPCA1-1171
Catalog No. ABIN6941172
$593.17
Plus shipping costs $45.00
100 μg ABIN6941172
100 μg ABIN6941172
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  • Target
    Reactivity
    Human, Rat
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This CD34 antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
    Specificity
    This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant full-length human HPCA1 protein
    Clone
    HPCA1-1171
    Isotype
    IgG1, kappa
  • Application Notes

    Positive Control: KG-1 cells. Human tonsil or angiosarcoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Alternative Name
    CD34 (CD34 Antibody Abstract)
    Molecular Weight
    90-110kDa
    Gene ID
    947
    UniProt
    P28906
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