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dsDNA antibody

dsDNA Reactivity: Human IF, FACS, IHC, StM Host: Mouse Monoclonal 121-3 unconjugated
Catalog No. ABIN6941357
  • Target See all dsDNA products
    dsDNA (Double-Stranded DNA (dsDNA))
    Reactivity
    • 14
    • 2
    • 1
    Human
    Host
    • 15
    • 2
    Mouse
    Clonality
    • 15
    • 2
    Monoclonal
    Conjugate
    • 10
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This dsDNA antibody is un-conjugated
    Application
    • 12
    • 11
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes, DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly associated pair of molecules in the shape of a right-handed double helix. Hydrogen bonds as well as forces generated by the hydrophobic effect and pi stacking hold the two DNA strands together. During replication and transcription, portions of the helix unwind and become single stranded. Protective proteins surround these single-stranded DNA. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
    Immunogen
    Nuclei of Burkitt's cells
    Clone
    121-3
    Isotype
    IgG3 kappa
  • Application Notes

    Positive Control: Raji, Jurkat or HeLa cells. Tonsil or Colon.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    dsDNA (Double-Stranded DNA (dsDNA))
    Alternative Name
    Double Stranded DNA (dsDNA) (Nuclear Marker) (dsDNA Products)
    Target Type
    Chemical
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