Human Papilloma Virus 16, 18 E6 (HPV-16, HPV-18 E6) antibody

Catalog No. ABIN6941439

Product Details

Target: Human Papilloma Virus 16, 18 E6 (HPV-16, HPV-18 E6)

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Coating (Coat)

Purification: Purified by Protein A/G

Immunogen: HPV18 E6-β-galactosidase fusion protein

Clone: HPV16-1295-HPV18-1297

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Human cervical cancer cells. Cervical tissue.

Known Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Human Papilloma Virus 16, 18 E6 (HPV-16, HPV-18 E6)

Alternative Name: HPV-16 E6 + HPV-18 E6 (Human Papilloma Virus 16/18)

Target Type: Viral Protein

Background: Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90 % of cervical cancers contain HPV DNA of the high risk types. Mutational analysis has shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Molecular Weight: 16/17kDa