CHEK1 antibody

Catalog No. ABIN6942550

The Rabbit Monoclonal anti-CHEK1 antibody has been validated for WB, IHC, IHC (p), IF (cc) and FACS. It is suitable to detect CHEK1 in samples from Human and Mouse.

Quick Overview for CHEK1 antibody (ABIN6942550)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Clone: 1A1

Product Details anti-CHEK1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: C-terminus human Chk1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Chk1

Background:

Synonyms: Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011].

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair