H2AFX antibody (AA 1-50)

Catalog No. ABIN6943600

The Rabbit Monoclonal anti-H2AFX antibody is suitable to detect H2AFX in samples from Human. It has been validated for WB, IHC (p), IF (p) and IF (cc).

Quick Overview for H2AFX antibody (AA 1-50) (ABIN6943600)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 9C9

Product Details anti-H2AFX Antibody

Binding Specificity: AA 1-50

Purpose: Histone H2AX (9C9) Monoclonal Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic peptide within human Histone H2A.X aa 1-50

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX

Background:

Synonyms: Histone H2A.X, Histone H2AX, H2a/x, H2AX, H2AFX., H2A histone family member X.

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Gene ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus