Nibrin antibody (NBN)

Details for Product anti-NBN Antibody No. ABIN6944404
Antigen
  • NBN
  • AT-V1
  • AT-V2
  • ATV
  • NBS
  • NBS1
  • P95
  • Nbs1
  • im:6911679
  • zgc:194152
  • nibrin
  • NBN
  • nbn
  • Nbn
Reactivity
Human, Mouse (Murine), Rat (Rattus)
313
111
90
5
1
1
1
1
Host
Rabbit
245
74
Clonality (Clone)
Monoclonal ()
Conjugate
This Nibrin antibody is un-conjugated
12
8
8
8
8
8
8
5
3
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Western Blotting (WB)
236
97
83
52
47
26
24
17
14
8
7
3
2
2
1
1
Options
Clone 3G5
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others Nibrin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name p95 NBS1 (NBN Antibody Abstract)
Background

Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.


Synonyms: Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, NBN
Gene ID 4683
UniProt O60934
Pathways DNA Damage Repair, Production of Molecular Mediator of Immune Response
Application Notes WB(1:300-1000), FCM(1:20-100), IF(ICC)(1:50-200)
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
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