Recombinant RAGE antibody (AA 1-350)

Catalog No. ABIN6944740

The Rabbit Monoclonal anti-RAGE antibody is suitable to detect RAGE in samples from Mouse and Rat. It has been validated for WB, IHC, FACS, IHC (p) and IF (cc).

Quick Overview for Recombinant RAGE antibody (AA 1-350) (ABIN6944740)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Antibody Type: Recombinant Antibody

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAGE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-RAGE Antibody

Binding Specificity: AA 1-350

Purpose: RAGE Recombinant Antibody

Cross-Reactivity: Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Human RAGE between 1 to 350 amino acids

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200), IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: RAGE

Background:

Synonyms: Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER

Background: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.

Gene ID: 177

UniProt: Q15109

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins