Recombinant GFP antibody (Alexa Fluor 647)

Catalog No. ABIN6953232

This Alpaca Monoclonal antibody specifically detects GFP in IF, ICC and FACS. It exhibits reactivity toward Aequorea victoria.

Quick Overview for Recombinant GFP antibody (Alexa Fluor 647) (ABIN6953232)

Target: GFP (Green Fluorescent Protein (GFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Aequorea victoria

Host: Alpaca

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Alexa Fluor 647

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1H1-1B2

Product Details anti-GFP Antibody (Alexa Fluor 647)

Expression System: E.coli

Purpose: Camelid sdAb anti-GFP conjugated with Alexa647, Clones 1H1/1B2

Specificity: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Cross-Reactivity (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Labeling Ratio: A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.

Application Details

Application Notes: Recommended dilution 1:500

Comment:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Concentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Expiry Date: 6 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP