CD45RO antibody (Biotin)
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- Target See all CD45RO products
- CD45RO
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD45RO antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Chimpanzee
- Purification
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted biotin removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- Clone
- UCHL1
- Isotype
- IgG2a kappa
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- Application Notes
- This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
- Comment
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0.5 mg/mL
- Restrictions
- For Research Use only
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- 2-8°C protected from light
- Expiry Date
- 12 months
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- Target
- CD45RO
- Alternative Name
- CD45RO (CD45RO Products)
- Background
- The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell- specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB.The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages and granulocytes. The antibody is reported to be cross-reactive for Chimpanzee CD45RO.
- Gene ID
- 5788
- UniProt
- P08575
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