Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

MHC II (I-A/I-E) antibody (Biotin)

Reactivity: Mouse FACS Host: Rat Monoclonal M5-114-15-2 Biotin
Catalog No. ABIN7278610
  • Target See all MHC II (I-A/I-E) products
    MHC II (I-A/I-E)
    Reactivity
    Mouse
    Host
    • 23
    Rat
    Clonality
    • 23
    Monoclonal
    Conjugate
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MHC II (I-A/I-E) antibody is conjugated to Biotin
    Application
    • 23
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Purification
    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted biotin removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
    Clone
    M5-114-15-2
    Isotype
    IgG2b kappa
  • Application Notes
    This antibody preparation has been quality-tested for flow cytometry using an appropriate cell type (as indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
    Comment

    0.5 mg/mL

    Restrictions
    For Research Use only
  • Buffer
    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    2-8°C protected from light
    Expiry Date
    12 months
  • Target
    MHC II (I-A/I-E)
    Alternative Name
    MHC Class II (I-A/I-E) (MHC II (I-A/I-E) Products)
    Background
    The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
    Gene ID
    14961, 14969
    UniProt
    P14483
You are here:
Support