CSF1R antibody

Catalog No. ABIN6961453

The Rat Monoclonal anti-CSF1R antibody (Clone AFS98) (ABIN6961453) specifically detects CSF1R in WB, FACS and Func. The antibody is reactive with Mouse samples.

Quick Overview for CSF1R antibody (ABIN6961453)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CSF1R antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: AFS98

Product Details anti-CSF1R Antibody

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Endotoxin Level: Low endotoxin: < 0.01 EU/ug

Isotype: IgG2a kappa

Application Details

Application Notes: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Comment:

2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Preservative: Azide free

Storage: 4 °C

Storage Comment: 2-8°C

Expiry Date: 12 months

Target Details for CSF1R

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CD115 (c-fms)

Background: The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid pre- cursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteo- clasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor.

Gene ID: 12978

UniProt: P09581

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization