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TCR beta antibody (violetFluor™ 450)

TCR beta Reactivity: Mouse FACS Host: Armenian Hamster Monoclonal H57-597 violetFluor™ 450
Catalog No. ABIN7278574
  • Target See all TCR beta Antibodies
    TCR beta (T Cell Receptor (TCR) beta (TCR beta))
    Reactivity
    • 29
    • 15
    Mouse
    Host
    • 21
    • 11
    • 8
    • 4
    Armenian Hamster
    Clonality
    • 33
    • 11
    Monoclonal
    Conjugate
    • 13
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TCR beta antibody is conjugated to violetFluor™ 450
    Application
    • 33
    • 12
    • 7
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS)
    Purification
    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
    Clone
    H57-597
    Isotype
    IgG
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  • Application Notes
    This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.
    Comment

    0.2 mg/mL

    Restrictions
    For Research Use only
  • Buffer
    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    2-8°C protected from light
    Expiry Date
    12 months
  • Target
    TCR beta (T Cell Receptor (TCR) beta (TCR beta))
    Alternative Name
    TCR beta (TCR beta Products)
    Synonyms
    TCRbeta antibody, Tib antibody, T cell receptor beta chain antibody, Tcrb antibody
    Background
    The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can re- sult in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.
    Gene ID
    21577
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