IL-1 beta antibody (Biotin)

Catalog No. ABIN6963832

This anti-IL-1 beta antibody is a Mouse Monoclonal antibody detecting IL-1 beta in ELISA and ELISpot. Suitable for Human. This Primary Antibody has been cited in 4+ publications.

Quick Overview for IL-1 beta antibody (Biotin) (ABIN6963832)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: 7P10

Product Details anti-IL-1 beta Antibody (Biotin)

Specificity: Native and recombinant human IL-1β. The antibody binds to the pro-form and active form of IL-1β. No cross-reactivity with IL-1α.

Cross-Reactivity (Details): This monoclonal antibody enables sensitive and specific detection of human IL-1β in immunoassays such as ELISA and ELISpot. The antibody binds to the pro-form and the active form of IL-1β. It does not cross-react with IL-1α.

Characteristics: This monoclonal antibody enables specific detection of human IL-1β in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-1beta

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963832 and ABIN7448283 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963831 and ABIN7448282 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-1 beta antibody (Biotin) (ABIN6963832)

Palizgir, Akhtari, Mahmoudi, Mostafaei, Rezaiemanesh, Shahram: "Curcumin reduces the expression of interleukin 1β and the production of interleukin 6 and tumor necrosis factor alpha by M1 macrophages from patients with Behcet's disease." in: Immunopharmacology and immunotoxicology, Vol. 40, Issue 4, pp. 297-302, (2018) (PubMed).

Lang, Lee, Elgass, Pinar, Tate, Aitken, Fan, Creed, Deen, Traore, Mueller, Stanisic, Baiwog, Skene, Wilce, Mansell, Morand, Harris: "Macrophage migration inhibitory factor is required for NLRP3 inflammasome activation." in: Nature communications, Vol. 9, Issue 1, pp. 2223, (2018) (PubMed).

Chen, Messina, Germano, Bonnici, Freyne, Cheung, Goldsmith, Kollmann, Levin, Burgner, Curtis: "Innate immune responses following Kawasaki disease and toxic shock syndrome." in: PLoS ONE, Vol. 13, Issue 2, pp. e0191830, (2018) (PubMed).

Gille-Johnson, Smedman, Gudmundsdotter, Somell, Nihlmark, Paulie, Andersson, Gårdlund: "Circulating monocytes are not the major source of plasma cytokines in patients with sepsis." in: Shock (Augusta, Ga.), Vol. 38, Issue 6, pp. 577-83, (2013) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B

Gene ID: 3553

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome