TNF alpha antibody (Biotin)

Catalog No. ABIN6963897

The Rat Monoclonal anti-TNF alpha antibody has been validated for ELISA and ELISpot. It is suitable to detect TNF alpha in samples from Mouse. There are 5+ publications available.

Quick Overview for TNF alpha antibody (Biotin) (ABIN6963897)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: MT11B10

Product Details anti-TNF alpha Antibody (Biotin)

Specificity: Natvie and recombinant TNF-α

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant mouse TNF-alpha

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-TNF alpha antibody (Biotin) (ABIN6963897)

Chen, Chen, Ren, Xia, Zhu, Liu, Zhang, Tang, Sun, Liu, Dong: "Aberrant DNA methylation of mTOR pathway genes promotes inflammatory activation of immune cells in diabetic kidney disease." in: Kidney international, Vol. 96, Issue 2, pp. 409-420, (2020) (PubMed).

Li, Yan, Huang, Chi, Xu, Li, Liu, Feng, Wang, Zhao, Yang, Xia: "Characterization of the Immune Response of MERS-CoV Vaccine Candidates Derived from Two Different Vectors in Mice." in: Viruses, Vol. 12, Issue 1, (2020) (PubMed).

Shekar, Ramlal, Jeyabalaji, Sripathy: "Intranasal immunization of cocktail/fusion protein containing Tir along with ΔG active fragment of Zot as mucosal adjuvant confers enhanced immunogenicity and reduces E. coli O157:H7 shedding in mice." in: International immunopharmacology, Vol. 63, pp. 211-219, (2018) (PubMed).

Chida, Hara, Yano, Uchiyama, Das, Takahashi, Miyata, Tomioka, Ito, Kido, Sakaguchi: "Prion protein protects mice from lethal infection with influenza A viruses." in: PLoS pathogens, Vol. 14, Issue 5, pp. e1007049, (2018) (PubMed).

Abbassi-Daloii, Yousefi, Sekhavati, Tahmoorespur: "Impact of heat shock protein 60KD in combination with outer membrane proteins on immune response against Brucella melitensis." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 126, Issue 1, pp. 65-75, (2018) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNFA

Gene ID: 21926

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome