TNF alpha antibody

Catalog No. ABIN6963899

This Mouse Monoclonal antibody specifically detects TNF alpha in ELISA and ELISpot. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for TNF alpha antibody (ABIN6963899)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, ELISpot

Clone: MT25C5

Product Details anti-TNF alpha Antibody

Specificity: Native and recombinant TNF-α

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human TNF-alpha

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-TNF alpha antibody (ABIN6963899)

Motedayyen, Fathi, Fasihi-Ramandi, Ali Taheri: "The effect of lipopolysaccharide on anti-inflammatory and pro-inflammatory cytokines production of human amniotic epithelial cells." in: Reproductive biology, Vol. 18, Issue 4, pp. 404-409, (2019) (PubMed).

Herrera, Tsai, Chang, Hamel, Wang, Lu, Mboup, Kanki: "Sustained Specific and Cross-Reactive T Cell Responses to Zika and Dengue Virus NS3 in West Africa." in: Journal of virology, Vol. 92, Issue 7, (2018) (PubMed).

Arachchi, Weerasekera, Senevirathna, Weerasekera, Fernando, Gunasekara: "Association of tumor necrosis factor alpha gene polymorphisms with Helicobacter pylori infection in dyspeptic patients in Sri Lanka." in: Microbiology and immunology, (2018) (PubMed).

Gille-Johnson, Smedman, Gudmundsdotter, Somell, Nihlmark, Paulie, Andersson, Gårdlund: "Circulating monocytes are not the major source of plasma cytokines in patients with sepsis." in: Shock (Augusta, Ga.), Vol. 38, Issue 6, pp. 577-83, (2013) (PubMed).

Snyder-Cappione, Tincati, Eccles-James, Cappione, Ndhlovu, Koth, Nixon: "A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-α and MIP1-β, a lack of IL-17, and a Th1-bias in males." in: PLoS ONE, Vol. 5, Issue 11, pp. e15412, (2011) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNFA

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome