TNF alpha antibody (Biotin)

Catalog No. ABIN6963900

The Mouse Monoclonal anti-TNF alpha antibody has been validated for ELISA and ELISpot. It is suitable to detect TNF alpha in samples from Human. There are 5+ publications available.

Quick Overview for TNF alpha antibody (Biotin) (ABIN6963900)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: MT20D9

Product Details anti-TNF alpha Antibody (Biotin)

Specificity: Native and recombinant TNF-α

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human TNF-alpha

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-TNF alpha antibody (Biotin) (ABIN6963900)

Herrera, Tsai, Chang, Hamel, Wang, Lu, Mboup, Kanki: "Sustained Specific and Cross-Reactive T Cell Responses to Zika and Dengue Virus NS3 in West Africa." in: Journal of virology, Vol. 92, Issue 7, (2018) (PubMed).

Bao, Wang, Liu, Shi, Lu, Sha, Weng, Hanabuchi, Qin, Plumas, Chaperot, Zhang, Liu: "NFATC3 promotes IRF7 transcriptional activity in plasmacy--toid dendritic cells." in: The Journal of experimental medicine, Vol. 213, Issue 11, pp. 2383-2398, (2017) (PubMed).

McMahon, Banville, Bergin, Smedman, Paulie, Reeves, Kavanagh: "Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins." in: Inflammation, Vol. 39, Issue 1, pp. 425-433, (2016) (PubMed).

Sundström, Nguyen Hoang, Gupta, Ahlm, Svensson, Klingström: "Andes Hantavirus-Infection of a 3D Human Lung Tissue Model Reveals a Late Peak in Progeny Virus Production Followed by Increased Levels of Proinflammatory Cytokines and VEGF-A." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149354, (2016) (PubMed).

Snyder-Cappione, Tincati, Eccles-James, Cappione, Ndhlovu, Koth, Nixon: "A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-α and MIP1-β, a lack of IL-17, and a Th1-bias in males." in: PLoS ONE, Vol. 5, Issue 11, pp. e15412, (2011) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNFA

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome