IL17A
Reactivity: Human
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Detection of IL-17A producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-17A by flow cytometry in human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-human IL-17A mAb MT504-FITC and anti-human IFN-γ-PE (right panel). The left panel depicts stimulated cells stained with matched isotype control antibodies. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a BD FACSVerse system.
Restrictions
For Research Use only
Format
Liquid
Buffer
in PBS with 0.2 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.