IL-21 antibody (Biotin)

Catalog No. ABIN6963916

Product Details anti-IL-21 Antibody (Biotin)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-21 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Specificity: Native and recombinant human IL-21

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-21 from non-human primates. See the Human/NHP cross-reactivity table.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-21

Clone: MT21-3m

Isotype: IgG1

Application Details

Application Notes: For quantification of IL-21 in solution e.g. cell culture supernatants using ELISA and for enumeration of IL-21 producing cells using ELISpot. MT21.3m is recommended as detection mAb in combination with coating mAb MT216G . The antibody is also suitable for Western blot.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-21 antibody (Biotin) (ABIN6963916)

Yamamoto, Kitawaki, Sugimoto, Fujita, Kawase, Takaori-Kondo, Kadowaki: "Anti-inflammatory modulation of human myeloid-derived dendritic cell subsets by lenalidomide." in: Immunology letters, Vol. 211, pp. 41-48, (2020) (PubMed).

Amu, Lantto Graham, Bekele, Nasi, Bengtsson, Rethi, Sorial, Meini, Zazzi, Hejdeman, Chiodi: "Dysfunctional phenotypes of CD4+ and CD8+ T cells are comparable in patients initiating ART during early or chronic HIV-1 infection." in: Medicine, Vol. 95, Issue 23, pp. e3738, (2017) (PubMed).

Li, Sullivan, Rouphael, Yu, Banton, Maddur, McCausland, Chiu, Canniff, Dubey, Liu, Tran, Hagan, Duraisingham, Wieland, Mehta, Whitaker, Subramaniam, Jones, Sette, Vora, Weinberg, Mulligan, Nakaya et al.: "Metabolic Phenotypes of Response to Vaccination in Humans. ..." in: Cell, Vol. 169, Issue 5, pp. 862-877.e17, (2017) (PubMed).

Huang, Ehrnfelt, Paulie, Zuber, Ahlborg: "ELISpot and ELISA analyses of human IL-21-secreting cells: Impact of blocking IL-21 interaction with cellular receptors." in: Journal of immunological methods, Vol. 417, pp. 60-66, (2015) (PubMed).

Target Details for IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL21 (IL21 Products)

Synonyms: IL-21 antibody, Za11 antibody, interleukin 21 antibody, IL21 antibody, Il21 antibody

Gene ID: 59067

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response