H2AFX antibody (pTyr142)

Catalog No. ABIN6971768

This Rabbit Polyclonal antibody specifically detects H2AFX in WB and DB. It exhibits reactivity toward Human.

Quick Overview for H2AFX antibody (pTyr142) (ABIN6971768)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details anti-H2AFX Antibody

Binding Specificity: pTyr142

Characteristics: Histone H2A.XY142ph (H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. Tyrosine phosphorylation at position 142 on Histone H2A.X is constitutive and is mediated by BAZ1B/WSTF. This mark distinguishes between apoptotic and DNA repair responses to genotoxic stress. Histone H2A.XY142ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide containing phospho-tyrosine 142 of human histone H2A.X.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: Histone H2A.X

Molecular Weight: 15 kDa

NCBI Accession: NP_002096

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus