Histone H2B antibody

Catalog No. ABIN6971801

This Rabbit Polyclonal antibody specifically detects Histone H2B in WB. It exhibits reactivity toward Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Quick Overview for Histone H2B antibody (ABIN6971801)

Target: Histone H2B

Reactivity: Saccharomyces cerevisiae, Schizosaccharomyces pombe

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H2B Antibody

Characteristics: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2B antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Budding Yeast and Fission Yeast samples.

Purification: Protein A Chromatography

Immunogen: This yeast histone H2B antibody was raised against recombinant yeast histone H2B.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H2B

Target: Histone H2B

Molecular Weight: 14 kDa

NCBI Accession: NP_010510