MYF5 antibody

Catalog No. ABIN6972389

This Rat Monoclonal antibody specifically detects MYF5 in WB, IF and ICC. It exhibits reactivity toward Mouse.

Quick Overview for MYF5 antibody (ABIN6972389)

Target: MYF5 (Myogenic Factor 5 (MYF5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MYF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1E2G4

Product Details anti-MYF5 Antibody

Characteristics: Myf-5 (Myogenic Factor 5, MFY5) is a basic helix-loop-helix transcription factor involved in muscle differentiation. Myf-5 induces differentiation of fibroblasts into myoblasts. Efficient DNA binding requires dimerization with another bHLH protein. Myf-5 antibody (mAb) (Clone 1E2G4) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Mouse samples.

Purification: Protein G Chromatography

Immunogen: This Myf-5 antibody was raised against a peptide derived from mouse Myf-5.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Alternative Name: Myf-5

Molecular Weight: 28 kDa

NCBI Accession: NP_032682

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development