POLR2F antibody

Catalog No. ABIN6972612

Product Details anti-POLR2F Antibody

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Characteristics: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. RNA pol II antibody (mAb) (Clone 1F4B6) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.

Clone: 1F4B6

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA pol II (POLR2F Products)

Synonyms: HRBP14.4 antibody, POLRF antibody, RPABC2 antibody, RPB14.4 antibody, RPB6 antibody, 1810060D16Rik antibody, RNA polymerase II subunit F antibody, polymerase (RNA) II (DNA directed) polypeptide F antibody, POLR2F antibody, Polr2f antibody

Molecular Weight: 240 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways