RNA Pol II CTD Ser2ph / Ser5ph (pSer2), (pSer5) antibody
Reactivity: Human
ICC, IF, WB, ChIP, ChIP-seq
Host: Mouse
Monoclonal
1A12G10
unconjugated
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- Target
- RNA Pol II CTD Ser2ph / Ser5ph
- Binding Specificity
- pSer2, pSer5
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)
- Characteristics
- RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 2 phosphorylation is enriched over the gene body and is associated with transcriptional elongation. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.
- Purification
- Protein A Chromatography
- Immunogen
- This antibody was raised against a synthetic peptide containing phospho-serine 2 and phospho-serine 5 of the CTD of human RNA Polymerase II.
- Clone
- 1A12G10
- Isotype
- IgG1 kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Target
- RNA Pol II CTD Ser2ph / Ser5ph
- Molecular Weight
- 240 kDa
- NCBI Accession
- NP_000928
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