PAK1 antibody (pSer204) (AbBy Fluor® 594)

Catalog No. ABIN6979651

This Rabbit Polyclonal antibody specifically detects PAK1 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PAK1 antibody (pSer204) (AbBy Fluor® 594) (ABIN6979651)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PAK1 Antibody (AbBy Fluor® 594)

Binding Specificity: pSer204

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of Ser204

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background:

Synonyms: PAK1 (phospho S204), p-PAK1 (phospho S204), ADRB2, Alpha PAK, Alpha-PAK, MGC130000, MGC130001, p21 activated kinase 1, p21 protein (Cdc42/Rac) activated kinase 1, p21-activated kinase 1, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related), p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p65 PAK, p65-PAK, P68-PAK, PAK alpha, PAK-1, Pak1, PAK1_HUMAN, Paka, PAKalpha, Protein kinase MUK2, Rac/p21-activated kinase, Serine/threonine-protein kinase PAK 1, STE20 homolog yeast.

Background: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Gene ID: 5058

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis