CHEK1 antibody (pSer296)

Catalog No. ABIN6989950

This Rabbit Monoclonal antibody specifically detects CHEK1 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CHEK1 antibody (pSer296) (ABIN6989950)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4B6

Product Details anti-CHEK1 Antibody

Binding Specificity: pSer296

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Synthetic phospho-Peptide corresponding to residues surrounding Ser296 of human Chk1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Chk1

Background:

Synonyms: C85740 antibody, Cell cycle checkpoint kinase antibody, Checkpoint , S. pombe, homolog of, 1 antibody, Checkpoint kinase 1 antibody, Checkpoint kinase 1 homolog (S. pombe) antibody, CHEK 1 antibody, Chek1 antibody, Chk 1 antibody, Chk1 antibody, CHK1 checkpoint homolog (S. pombe) antibody, CHK1_HUMAN antibody, EC 2.7.11.1 antibody, rad27 antibody, Serine/threonine protein kinase Chk1 antibody, Serine/threonine-protein kinase CHK1 antibody, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011].

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair