PUMA antibody (AA 76-170)

Catalog No. ABIN6990173

The Mouse Monoclonal anti-PUMA antibody has been validated for WB and ELISA. It is suitable to detect PUMA in samples from Human and Rat.

Quick Overview for PUMA antibody (AA 76-170) (ABIN6990173)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2A8F6

Product Details anti-PUMA Antibody

Binding Specificity: AA 76-170

Purification: PUMA Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Immunogen: Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.

Isotype: IgG1

Application Details

Application Notes: PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PUMA Monoclonal Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: PUMA monoclonal antibody can be stored at -20°C, stable for one year.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα, and PUMAβ,. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity