MPRIP antibody (C-Term)

Catalog No. ABIN6990325

Product Details anti-MPRIP Antibody

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPRIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: RIP3 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-RIP3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3.

Isotype: IgG

Application Details

Application Notes: WB: 0.1-0.5 μ,g/mL, IHC-P: 5 μ,g/mL, IF: 20 μ,g/mL, IP: 20 μ,g/mL.

Antibody validated: Western Blot in mouse and human samples, Immunohistochemistry in mouse and rat samples, Immunofluorescence in mouse and rat samples, Immunoprecipitation in human samples, Immunogold EM in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RIP3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RIP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

References for anti-MPRIP antibody (ABIN6990325)

Wu, Chung, Cao, Jin, Edmiston, Hickman, Levy, Whalen, Abrams, Degterev, Lo, Tozzi, Kaplan, El Khoury, Whalen: "Genetic inhibition of RIPK3 ameliorates functional outcome in controlled cortical impact independent of necroptosis." in: Cell death & disease, Vol. 12, Issue 11, pp. 1064, (2021) (PubMed).

Target Details for MPRIP

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Alternative Name: RIP3 (MPRIP Products)

Synonyms: M-RIP antibody, MRIP antibody, RHOIP3 antibody, RIP3 antibody, p116Rip antibody, 9530046C02 antibody, AA536749 antibody, AI647711 antibody, C76423 antibody, Rhoip3 antibody, mKIAA0864 antibody, M-rip antibody, Mrip antibody, myosin phosphatase Rho interacting protein antibody, MPRIP antibody, Mprip antibody

Background: RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κ,B activation. Overexpression of RIP3 induces apoptosis and NF-κ,B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

Molecular Weight: Predicted: 53kD for mouse RIP3 and 57kD for human RIP3

Observed: 53kD for mouse RIP3 and 57kD for human RIP3 kDa

Gene ID: 56532

UniProt: Q9QZL0