MPRIP antibody (Internal Region)

Catalog No. ABIN7216892

The Rabbit Polyclonal anti-MPRIP antibody (ABIN7216892) specifically detects MPRIP in WB, IHC and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MPRIP antibody (Internal Region) (ABIN7216892)

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPRIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MPRIP Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-M-RIP Polyclonal Antibody

Specificity: M-RIP Polyclonal Antibody detects endogenous levels of M-RIP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human M-RIP

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MPRIP

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Alternative Name: M-RIP

Background: MPRIP, KIAA0864, MRIP, RHOIP3, Myosin phosphatase Rho-interacting protein, M-RIP, Rho-interacting protein 3, RIP3, p116RipMPRIP (Myosin Phosphatase Rho Interacting Protein) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include actin binding. Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.

Molecular Weight: 115kD

Gene ID: 23164

UniProt: Q6WCQ1