PUMA antibody (Isoform 1, N-Term)

Catalog No. ABIN6990344

The Rabbit Polyclonal anti-PUMA antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect PUMA in samples from Human and Rat.

Quick Overview for PUMA antibody (Isoform 1, N-Term) (ABIN6990344)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PUMA Antibody

Binding Specificity: Isoform 1, N-Term

Purification: PUMA Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-PUMA antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PUMA isoform 1. The immunogen is located within the first 50 amino acids of PUMA.

Isotype: IgG

Application Details

Application Notes: WB: 2-3 μ,g/mL, IF: 10-20 μ,g/mL, IHC: 2.5-10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PUMA Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PUMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1). The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Molecular Weight: Predicted: 21kD

Observed: 23kD kDa

Gene ID: 27113

NCBI Accession: NP_055232

UniProt: Q96PG8

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity