PUMA antibody (C-Term, Isoform 1)

Catalog No. ABIN6990494

The Rabbit Polyclonal anti-PUMA antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect PUMA in samples from Human and Mouse.

Quick Overview for PUMA antibody (C-Term, Isoform 1) (ABIN6990494)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PUMA Antibody

Binding Specificity: C-Term, Isoform 1

Specificity: A lower band at approximately 16 kDa was detected in Daudi and K562 cells, which may represent the PUMA isoform 2.

Purification: PUMA Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-PUMA antibody was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus human PUMA isoform 1. The immunogen is located within the last 50 amino acids of PUMA.

Isotype: IgG

Application Details

Application Notes: WB: 1-4 μ,g/mL, IF: 2 μ,g/mL, ICC: 1 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PUMA Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PUMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1). The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Molecular Weight: 23 kDa

Gene ID: 27113

NCBI Accession: NP_055232

UniProt: Q96PG8

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity