Tuberin antibody (N-Term)

Catalog No. ABIN6990538

This Rabbit Polyclonal antibody specifically detects Tuberin in ELISA, WB, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for Tuberin antibody (N-Term) (ABIN6990538)

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Tuberin Antibody

Binding Specificity: N-Term

Purification: TSC2 Antibody is affinity chromatography purified via peptide column.

Immunogen: TSC2 (NT) was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TSC2. The immunogen is located within the first 50 amino acids of TSC2.

Isotype: IgG

Application Details

Application Notes: TSC2 antibody can be used for the detection of TSC2 by Western blot at 2 and 4 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TSC2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TSC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: TSC2

Background: TSC2 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Gene ID: 7249

NCBI Accession: NP_000539

UniProt: P49815

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus