BCL2A1 antibody (C-Term)

Catalog No. ABIN6990657

Product Details anti-BCL2A1 Antibody

Target: BCL2A1 (BCL2-Related Protein A1 (BCL2A1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL2A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least two isoforms of Bfl-1 are known to exist, this antibody will only detect the larger isoform.

Purification: Bfl-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1. The immunogen is located within the last 50 amino acids of Bfl-1.

Isotype: IgG

Application Details

Application Notes: Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Bfl-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Bfl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BCL2A1

Target: BCL2A1 (BCL2-Related Protein A1 (BCL2A1))

Alternative Name: Bfl-1 (BCL2A1 Products)

Synonyms: ACC-1 antibody, ACC-2 antibody, BCL2L5 antibody, BFL1 antibody, GRS antibody, HBPA1 antibody, Bcl2a1d antibody, BCL2A1 antibody, BCL2 related protein A1 antibody, BCL2-related protein A1 antibody, BCL2A1 antibody, Bcl2a1 antibody

Background: Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells.

Molecular Weight: Predicted: 19 kDa

Observed: 20 kDa

Gene ID: 597

NCBI Accession: NP_004040

UniProt: Q16548