TANK antibody (C-Term)

Catalog No. ABIN6990659

The Rabbit Polyclonal anti-TANK antibody is suitable to detect TANK in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for TANK antibody (C-Term) (ABIN6990659)

Target: TANK (TRAF Family Member-Associated NFKB Activator (TANK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TANK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TANK Antibody

Binding Specificity: AA 350-400, C-Term

Purification: TANK Antibody is affinity chromatography purified via peptide column.

Immunogen: TANK antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TANK. The immunogen is located within amino acids 350 - 400 of TANK.

Isotype: IgG

Application Details

Application Notes: TANK antibody can be used for the detection of TANK by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TANK Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TANK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TANK

Target: TANK (TRAF Family Member-Associated NFKB Activator (TANK))

Alternative Name: TANK

Background: TANK Antibody: TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-κ,B. This activation of NF-κ,B occurs through an association with the kinases IKKε, and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the Iκ,B complex. This suggests that in addition to the possibility that TBK1 and IKKε, activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-κ,B proteins. At least two isoforms of TANK are known to exist.

Molecular Weight: Predicted: 47 kDa

Observed: 50 kDa

Gene ID: 10010

NCBI Accession: NP_004171

UniProt: Q92844

Pathways: p53 Signaling, TLR Signaling, Activation of Innate immune Response