MAVS antibody (AA 130-180)

Catalog No. ABIN6990722

Product Details anti-MAVS Antibody

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Binding Specificity: AA 130-180

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAVS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: VISA Antibody is affinity chromatography purified via peptide column.

Immunogen: VISA antibody was raised against a 17 amino acid synthetic peptide from near the center of human VISA. The immunogen is located within amino acids 130 - 180 of VISA.

Isotype: IgG

Application Details

Application Notes: VISA antibody can be used for detection of VISA by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VISA Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: VISA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MAVS

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Alternative Name: VISA (MAVS Products)

Synonyms: CARDIF antibody, IPS-1 antibody, IPS1 antibody, VISA antibody, D430028G21Rik antibody, Visa antibody, cardif antibody, wu:fj20d04 antibody, zgc:158392 antibody, mitochondrial antiviral signaling protein antibody, MAVS antibody, Mavs antibody, mavs antibody

Background: VISA Antibody: Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-κ,B. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKα, IKKβ, and IKKε, kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.

Gene ID: 57506

NCBI Accession: NP_065797

UniProt: Q7Z434

Pathways: Activation of Innate immune Response, Inositol Metabolic Process, Hepatitis C