TARDBP antibody (N-Term)

Catalog No. ABIN6990775

This Rabbit Polyclonal antibody specifically detects TARDBP in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TARDBP antibody (N-Term) (ABIN6990775)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-TARDBP Antibody

Binding Specificity: AA 60-110, N-Term

Specificity: At least two isoforms are known to exist for this protein, this TDP43 antibody will recognize both isoforms.

Purification: TDP43 Antibody is affinity chromatography purified via peptide column.

Immunogen: TDP43 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TDP43. The immunogen is located within amino acids 60 - 110 of TDP43.

Isotype: IgG

Application Details

Application Notes: TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TDP43 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TDP43 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TDP43

Background: TDP43 Antibody: TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3'splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

Gene ID: 23435

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion