Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Purification
CHORDC1 Antibody is affinity chromatography purified via peptide column.
Immunogen
CHORDC1 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human CHORDC1. The immunogen is located within amino acids 50 - 100 of CHORDC1.
CHORDC1
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 647
Application Notes
CHORDC1 antibody can be used for detection of CHORDC1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
CHORDC1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
CHORDC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
CHORDC1
(Cysteine and Histidine-Rich Domain (CHORD)-Containing 1 (CHORDC1))
CHP1 antibody, CHP-1 antibody, MGC89160 antibody, NV16801 antibody, Chp-1 antibody, Morgana antibody, 1110001O09Rik antibody, AA409036 antibody, morgana antibody, zgc:63894 antibody, cysteine and histidine rich domain containing 1 antibody, cysteine and histidine-rich domain-containing protein antibody, cysteine and histidine rich domain containing 1 S homeolog antibody, cysteine and histidine-rich domain (CHORD)-containing 1 antibody, cysteine and histidine-rich domain (CHORD)-containing, zinc-binding protein 1 antibody, cysteine and histidine-rich domain (CHORD) containing 1b antibody, CHORDC1 antibody, Chordc1 antibody, LOC412067 antibody, chordc1.S antibody, chordc1 antibody, chordc1b antibody
Background
CHORDC1 Antibody: The cysteine and histidine-rich domain (CHORD)-containing protein (CHORDC1) is a member of a highly conserved protein family that contains the plant protein RAR1 and the mammalian protein melusin. In mammals, CHORDC1 is an ADP-dependent HSP90-interacting protein, and this interaction is dependent on the ability of HSP90 to bind nucleotides. Recent experiments indicate that CHORDC1 mRNA is diurnally regulated in mouse hypothalamus, and that this regulation alters during development, suggesting that CHORDC1 may play a role in circadian mechanisms in the mammalian brain.